Department of Floriculture and Dendrology

Responsible of subject: Assoc. Prof. Dr. Anikó Veres

Lecturer: Assist. Prof. Dr. István Dániel Mosonyi e-mail: mosonyi.istvan.daniel@uni-mate.hu

Credit value: 5

1+4 hours of week

Introduction to micropropagation and plant tissue culturing– theoretical backgrounds and practical methods. History of plant tissue culturing, plant regeneration from cells, tissues and organs, different developmental pathways – direct and indirect organogenesis and somatic embryogenesis. Biotechnology of sexual reproduction in plants. Opportunities and application in practice. Practical knowledge – media preparation, handling of laboratory equipment, starting and maintaining sterile shoot cultures, acclimation of in vitro plant material.

List of lectures and practical classes, requirements in the Spring Semester of 2023-2024. academic year (PDF)

Presentations of lectures:

• Introduction to the course, History of plant biotechnology (PDF)
• Plant -cell -plant system and micropropagation (PDF)
• Factors influencing in vitro cultures - sterility (PDF)
• Factors influencing in vitro cultures - media (PDF)
• Growth control effects of the physical environment (PDF)
• Stages and methodes of micropropagation (PDF)
• Economical aspects of micropropagation (PDF)
• Micropropagation laboratory equipment and design (PDF)
• Biotechnology of sexual reproduction (PDF)
• Micropropagation of orchids (PDF)
• Micropropagation of woody and herbaceous ornamental plants (PDF)
• Stages and methods of micropropagation (PDF)
• Factors influencing in vitro cultures media (PDF)
• Changes in plants during tissue culturing (PDF)